ABSTRACT
The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
Subject(s)
Animals , Molecular Diagnostic Techniques/methods , Penaeidae/virology , Real-Time Polymerase Chain Reaction/methods , Veterinary Medicine/methods , Sensitivity and SpecificityABSTRACT
This is the first report of outbreaks of Streptococcus iniae in Nile tilapia farms in South America. Seven isolates were identified by biochemical, serological and molecular tests. Their 16S rRNA gene sequences showed 100% similarity with S. iniae ATCC 29178 and two distinct PFGE patterns were observed for Brazilian isolates.
Subject(s)
Animals , Cichlids , Disease Outbreaks , Disease Susceptibility , Phylogeny , RNA , Polymerase Chain Reaction/methods , Sequence Analysis, DNA , Streptococcal Infections , Streptococcus/isolation & purification , Virulence Factors , Electrophoresis , Methods , VirulenceABSTRACT
The aim of this work was to evaluate the antibacterial activity of Brazilian plants extracts against fish pathogenic bacteria. Forty six methanolic extracts were screened to identify their antibacterial properties against Streptococcus agalactiae, Flavobacterium columnare and Aeromonas hydrophila. Thirty one extracts showed antibacterial activity.
O objetivo deste trabalho foi avaliar a atividade antibacteriana de extratos de plantas brasileiras contra bactérias patogênicas para peixes. A atividade antibacteriana de quarenta e seis extratos metanólicos de plantas foi avaliada contra os agentes Streptococcus agalactiae, Flavobacterium columnare e Aeromonas hydrophila. Trinta e um extratos apresentaram atividade antibacteriana.
Subject(s)
Aeromonas hydrophila , Anti-Bacterial Agents , Bacteria , Fishes , Flavobacterium , Plant Extracts , Streptococcus agalactiae , Methods , Methods , VirulenceABSTRACT
Caracterizaram-se as populações bacterianas isoladas de três sistemas de cultivo de tilápias, sem utilização prévia de antimicrobianos. No sistema 1 usou-se tanque de alvenaria com arraçoamento, no sistema 2, tanque de terra com arraçoamento e no sistema 3, tanque de terra com adubação orgânica. Posteriormente, foi verificado o perfil qualitativo de resistência a antimicrobianos de 98 amostras bacterianas. Membros da família Vibrionaceae predominaram nos três sistemas analisados. Observou-se elevado número de bactérias resistentes principalmente à ampicilina e à eritromicina. Bactérias resistentes à norfloxacina e à gentamicina não foram freqüentes e cerca de 50 por cento das amostras dos isolados bacterianos foram resistentes à tetraciclina. Dentre as amostras testadas, 96 por cento apresentaram resistência simultânea a dois ou mais antimicrobianos (MAR>0,2). O índice MAR médio para os três sistemas de criação foi 0,4, e foram mesmo considerados fontes de risco para disseminação de bactérias resistentes
Bacterial populations from different tilapia culture system with no antimicrobial use were characterized. Concret pond and commercial feed, land pond and commercial feed, and land pond and animal manure were used in systems I, II and III, respectively. Ninety-eight bacterial strains were subjected to sensitivity testing. Members of Vibrionaceae were the most prevalent in all systems analysed. The most bacterial strains were resistant to ampicillin and erithromicin, but resistance to norfloxacin and gentamicin were uncommon. A half to bacterial isolates was resistant to tetracycline. From the 98 bacterial isolates, 96 percent were resistant to two or more antimicrobials. The multiple antimicrobial resistance index was determined and it was similar for all systems analyzed (Mar= 0.4), indicating a high risk source for multiple antibiotic resistance